Detection of BlaIMP and BlaOXA-23-LIKE Genes in Acinetobacter  Baumannii Isolates at Dr. Wahidin Sudirohusodo Hospital

Authors

Acinetobacter baumannii, carbapenem resistant, carbapenem sensitive, blaIMP, blaOXA-23-like

Background/Objective: Carbapenem resistant Acinetobacter baumannii (CRAB) is an A. baumannii

resistant to one or more carbapenem antimicrobials. Some of resistance mechanisms that can be found

in CRAB are carbapenemase production, outer membran protein (OMP) or penicillin binding proteins

(PBP) structure modification, or efflux pump increase. Carbapenemase production can be affected by

carbapenemase-encoding genes such as blaIMP and blaOXA23-like gene. This study aimed to detect the blaIMP

and blaOXA23-like gene in A. baumannii isolates.

Material and Method : It was observational descriptive study with cross sectional approach. Subject of

this study were all A. baumannii isolates collected during the period of October 2018 until January 2019 at

Dr. Wahidin Sudirohusodo Hospital, Makassar, South Sulawesi, Indonesia (consisted of 30 CRAB and 20

sensitive carbapenem A. baumannii (CSAB) isolates). Isolates were examined by polymerase chain reaction

(PCR) and agar electrophoresis techniques.

Results : There were 5 CRAB isolates with blaIMP gene and 23 isolates with blaOXA23-like gene. There were

no CSAB isolates with blaIMP gene and 2 isolates with blaOXA23-like gene.

Conclusion : Carbapenemase-encoding genes could be found in CRABs and CSABs. In this study, blaOXA23-

like gene was found in both CSAB and CRAB isolates but blaIMP gene was only found in CRAB isolates.

Kartika Paramita1 , Tenri Esa, Benny Rusli1

1Department of Clinical Pathology, Faculty of Medicine, Hasanuddin University/Dr. Wahidin Sudirohusodo

Hospital, Makassar, South Sulawesi, Indonesia