Isolation of Bioactive Compounds and Free Radical  Scavenging Activity of Maclura Cochinchinensis (Lour.) Corner  Stem by Different Chromatographic Techniques

Authors

Muhammad Shariq Baber1 , Sakina Ruhi2 , Siti Aisyah Abd Rahman1 , Rasny M.R.M1 , Hamid A.K1 , Jiyauddin Khan1

Antioxidant, free radicals, bioactive compound, scavenging activity.

Antioxidants provide protection to human against free radicals through inhibiting and scavenging mechanism.

This study was to determine the free radical scavenging activity of Maclura cochinchinensis Corner stem

sample from different extraction methods to investigate the bioactive compound in stem extract. Materials

and methods: The stem samples of M. cochinchinensis were extracted by sonication, maceration and soxhlet

method with four different solvents; acetone, 50% ethanol, 80% ethanol and methanol. Each extracts was

tested using 2,2-diphenyl 1-picryl hydrazyl (DPPH) radical scavenging activity assay with ascorbic acid

and morin as positive control. Findings: For isolation of bioactive compound highest percentage yield of

the crude extracts was from 50% and 80% ethanol(22.0 ± 1.35 % and 20.7 ± 2.29 %), followed by acetone

(IC50 of 15.54 ± 1.34 µg/ml) and methanol (IC50 of 20.99 ± 1.07 µg/ml)extract by Soxhlet method not

significantly different, also had higher radicle scavenging activity, DPPH test on 80% ethanol extract from

soxhlet extraction was one of the highest antioxidant activity among the extracts.