Study of TP53 Gene-Codon 72 Polymorphism in Epithelial Ovarian Cancer (EOC)

Abstract

Introduction: The tumor-suppressor gene (TP53) encodes p53 protein, the vital protein in the apoptotic
cascade pathway which has been presented to be of crucial potential in the progression of cancers. Genetic
variation SNP that were revealed to affect the regulatory biochemical functions of p53 are the exon 4 codon
72, (G)Arg/(C)pro polymorphisms. Aim: the present study aims to analyses genetic variation (SNP) of Tp53
gene, codon 72 and p53 protein level in Epithelial Ovarian Cancer (EOC). Methods: the study achieved on 26
EOC cell line specimens and 26 specimens as control group, using PCR-RFLP genetic analysis method and
cell lysate p53 level determined by ELISA technique. Results: the percentage of allele polymorphism come
as homologous GG variant 85% and 23% in EOC specimens and control respectively and (CC) (8% and
46%) in EOC case and the control in order. Heterologous pattern CG was (34% and 23%) for experimental
and control group respectively.
Conclusion: our study proposes that 72 codon polymorphism (GG) is closely related to EOC progressions.