Molecular Diagnosis and Genotype Analysis of Giardia lambelia in Diarrheal Patient in Al-Diwaniyah Province-Iraq

Abstract

Three hundred and fifty-six stool samples were collected from the beginning of October 2019 till the end
of February 2020 from patients who suffering from gastrointestinal symptoms ,general stool examination
was done for detect the cysts and trophozoites of Giardia lamblia using direct smear and flotation method.
The results showed that 105 out of 356 samples were positive ( 29.49 %) . Nested PCR were done to the
sixty samples from the positive results microscopically selected randomly , triosephosphateisomerase (TPI)
gene that using in detection Giardia lamblia genotype shows result that positive samples of (A,B,E) while
not showed result positive to F . The current study also showed high significant association ( P <0.01) , and
noted that the genotype B is the most common than genotype A, where the number of infected samples with
the genetic pattern B 24/60 with percent 40% while the number of infected samples with genetic pattern A
18/60 with percent 30%.